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amplex red cholesterol assay kit  (Thermo Fisher)


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    Thermo Fisher amplex red cholesterol assay kit
    Differences in <t>cholesterol</t> removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.
    Amplex Red Cholesterol Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 8111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplex red cholesterol assay kit/product/Thermo Fisher
    Average 97 stars, based on 8111 article reviews
    amplex red cholesterol assay kit - by Bioz Stars, 2026-03
    97/100 stars

    Images

    1) Product Images from "Phospholipase A 2 products influence the antiplatelet functions of synthetic high-density lipoproteins"

    Article Title: Phospholipase A 2 products influence the antiplatelet functions of synthetic high-density lipoproteins

    Journal: Journal of Lipid Research

    doi: 10.1016/j.jlr.2025.100972

    Differences in cholesterol removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.
    Figure Legend Snippet: Differences in cholesterol removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.

    Techniques Used: Incubation, Amplex Red Cholesterol Assay, Labeling, Fluorescence, Control



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    Thermo Fisher amplex red cholesterol assay kit
    Differences in <t>cholesterol</t> removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.
    Amplex Red Cholesterol Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplex red cholesterol assay kit/product/Thermo Fisher
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    Differences in <t>cholesterol</t> removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.
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    Image Search Results


    Differences in cholesterol removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.

    Journal: Journal of Lipid Research

    Article Title: Phospholipase A 2 products influence the antiplatelet functions of synthetic high-density lipoproteins

    doi: 10.1016/j.jlr.2025.100972

    Figure Lengend Snippet: Differences in cholesterol removal capacity and cellular association of sHDL particles. (A) Washed human platelets were incubated with 22A peptide (100 μg/ml) and different sHDL particles (100 μg/ml of 22A peptide) for 15 min. The total cholesterol was quantified via Amplex Red Cholesterol Assay Kit. (B–E) Cellular associations of DiI-labeled sHDL particles (sHDLs at 100 μg/ml 22A, 200 μg/ml lipid, and 1 μg/ml DiI) following 5, 15, 30, and 60 min incubations with human washed platelets. Mean fluorescence intensity and representative histogram of DiI in platelets. Data represent mean ± SD (n = 5); ns = not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 versus control; #### P < 0.0001 versus 22A:POPC; §§ P < 0.01, §§§ P < 0.001, §§§§ P < 0.0001 versus 22A:DMPC.

    Article Snippet: The total cholesterol was determined using the Amplex Red Cholesterol Assay Kit (Invitrogen, ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s procedures.

    Techniques: Incubation, Amplex Red Cholesterol Assay, Labeling, Fluorescence, Control